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rabbit anti ddx3x antibody  (Proteintech)


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    Structured Review

    Proteintech rabbit anti ddx3x antibody
    Rabbit Anti Ddx3x Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ddx3x+antibody/DDX3+Antibody/pm41876452-196-9-15
    Average 94 stars, based on 62 article reviews
    rabbit anti ddx3x antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Arginine dependency in omental metastasis of epithelial ovarian cancer reveals a therapeutic vulnerability
    Article Snippet: The CUT&Tag (Cleavage Under Targets and Tagmentation) assay was performed using the AccuNext CUT&Tag Library Prep Kit for Illumina (Cat No. AG12556, Accurate Biology, China) according to the manufacturer’s instructions, with minor adaptations. .. Permeabilized cells were then sequentially incubated with a primary rabbit anti-DDX3X antibody (Cat. No. 11115-1-AP, Proteintech, Wuhan, China) and a secondary Goat Anti-Rabbit IgG antibody (Cat. No. AS070, ABclonal, Wuhan, China). ..

    other:

    Article Title: Arginine dependency in omental metastasis of epithelial ovarian cancer reveals a therapeutic vulnerability.
    Article Snippet: CUT&Tag AR TI CL E IN P RE SS The CUT&Tag (Cleavage Under Targets and Tagmentation) assay was performed using the AccuNext CUT&Tag Library Prep Kit for Illumina (Cat No. AG12556, Accurate Biology, China) according to the manufacturer's instructions, with minor adaptations.



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    U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced <t>DDX3X</t> protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained <t>using</t> <t>anti-DDX3X</t> antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.
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    U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced <t>DDX3X</t> protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained <t>using</t> <t>anti-DDX3X</t> antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.
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    U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced <t>DDX3X</t> protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained <t>using</t> <t>anti-DDX3X</t> antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.
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    U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced <t>DDX3X</t> protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained <t>using</t> <t>anti-DDX3X</t> antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.
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    U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced DDX3X protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained using anti-DDX3X antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Cell type-dependent suppression of the RNA helicase DDX3Y levels by the close paralog DDX3X

    doi: 10.3389/fcell.2026.1644807

    Figure Lengend Snippet: U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced DDX3X protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained using anti-DDX3X antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.

    Article Snippet: Primary antibodies used in this study include mouse anti-DDX3X (Santa Cruz sc-81247; 1:1,000), rabbit anti-DDX3X (Bethyl Laboratories A300-474A; 1:1,000), rabbit anti-DDX3X (Aviva OAAB01241; 1:1,000), rabbit anti-HA (Cell signaling Technology C29F4; 1:2,000), rabbit anti-GFP (Invitrogen A-11122,1:1,000) and custom-made rabbit DDX3Y antibody (1:2,000) ( ).

    Techniques: Generated, CRISPR, Mutagenesis, Western Blot, Transfection, Control

    U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced DDX3X protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained using anti-DDX3X antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Cell type-dependent suppression of the RNA helicase DDX3Y levels by the close paralog DDX3X

    doi: 10.3389/fcell.2026.1644807

    Figure Lengend Snippet: U87MG Clone 2 cells generated by CRISPR/Cas9 express reduced DDX3X protein. (A) Diagram of the genotyping results of Clone 2 and the putative encoded DDX3X protein. After the deletion of 14 nucleotides caused by CRISPR/Cas9, which was confirmed at both genomic DNA and cDNA levels, Clone 2 cells likely utilized a downstream translation start site to restore the open reading frame and prevent a complete loss of DDX3X protein. Residues in red were introduced by the mutation, while those in green were unchanged. (B) Representative western blots showing endogenous DDX3X in parental U87MG and Clone 2 cells (three biological replicated each), obtained using anti-DDX3X antibodies from two different sources. Quantification of the DDX3X band intensity detected with the Santa Cruz (SC) antibody is shown on the right. (C) Clone 2 cells were transfected with 100 nM control (CT), DDX3X and 3Y siRNA, and cell lysates were processed for western blotting with the SC antibody.

    Article Snippet: Primary antibodies used in this study include mouse anti-DDX3X (Santa Cruz sc-81247; 1:1,000), rabbit anti-DDX3X (Bethyl Laboratories A300-474A; 1:1,000), rabbit anti-DDX3X (Aviva OAAB01241; 1:1,000), rabbit anti-HA (Cell signaling Technology C29F4; 1:2,000), rabbit anti-GFP (Invitrogen A-11122,1:1,000) and custom-made rabbit DDX3Y antibody (1:2,000) ( ).

    Techniques: Generated, CRISPR, Mutagenesis, Western Blot, Transfection, Control